Browsing by Author "Bonaccorso, Frank J."
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Item A Test of Sex Specific Genetic Markers in the Hawaiian Hoary Bat and Relevance to Population Studies(2018-10-14) Pinzari, Corinna A.; Bonaccorso, Frank J.We tested the utility of a protocol using genetic markers that previously proved successful to identify the sex of Vespertilionid bats on tissues collected from live bats and carcasses of varying age from the Hawaiian hoary bat (Lasiurus cinereus semotus). This molecular method is based on genes unique to X and Y chromosomes in mammals and previously was used successfully on North American hoary bats (L. c. cinereus). We amplified two markers within intron regions of the zinc-finger-X (Zfx) and zinc-finger-Y (Zfy) genes using a multiplexed polymerase chain reaction technique and obtained product bands that were easily visualized using gel electrophoresis. Genotyping determined the sex of 36 individual Hawaiian hoary bat carcasses previously assigned sex only by external genitalia and identified sex for 29 “unknown” bat carcasses that could not be classified by external genitalia. Employing this method for sexing Hawaiian hoary bats will permit more reliable evaluation of the ratio of males to females in subpopulations affected by fatalities from emerging threats. This is critical to the conservation and management of this endangered bat.Item Acoustic surveys of Hawaiian hoary bats in Kahikinui Forest Reserve and Nakula Natural Area Reserve on the island of Maui(2016-07-08) Todd, Christopher M.; Pinzari, Corinna A.; Bonaccorso, Frank J.The Kahikinui Forest Reserve and the adjoining Nakula Natural Area Reserve (KFR-NNAR) was established in 2011 as a conservation area on the leeward slope of Haleakalā Volcano on the island of Maui to protect unique natural features and endangered species including the Hawaiian hoary bat, Lasiurus cinereus semotus. We recorded bat vocalizations from July 2012 to November 2014 using automated echolocation detectors at 14 point locations in the KFR-NNAR. Our study area included remnants of recovering mesic montane forest with interspersed grasses (1,250‒1,850 m elevation, hereafter called “forest”) and xeric subalpine shrubland plant communities (1,860‒2,800 m, hereafter called “shrubland”). Monthly detections of Hawaiian hoary bats, Lasiurus cinereus semotus, within the KFR-NNAR identified areas of high and low detection probability as well as foraging activity. Sixty per cent of all detector-nights had confirmed bat vocalizations and included detections in every month of the study. Monthly detection probability values were highest from July to November 2012; these values were significantly greater than values measured in any month thereafter. Pooled values of detection probabilities, mean pulses/night, percentage of nights with feeding activity, and acoustic detections all were greater in the recovering forest zone than corresponding values from the shrublands. Our data provide baseline levels of hoary bat echolocation activity that may be compared with future studies in the KFR-NNAR relative to success criteria for Hawaiian hoary bat habitat restoration.Item Hawaiian Hoary Bat (Lasiurus cinereus semotus) Acoustic Monitoring at Hawaii Army National Guard (HIARNG) Installations Statewide(2020-01-30) Montoya-Aiona, Kristina M.; Pinzari, Corinna A.; Peck, Robert W.; Brinck, Kevin W.; Bonaccorso, Frank J.Acoustic sampling for occurrence of the endangered Hawaiian hoary bat (Lasiurus cinereus semotus) was conducted at 18 “long-term” acoustic monitoring stations on 12 Hawai‘i Army National Guard (HIARNG) installations across the islands of Hawai‘i, Maui, Moloka‘i, O‘ahu, and Kaua‘i between 2012 and 2018. Bats were confirmed as present at 10 of these installations: Kealakekua Armory, Keaukaha Military Reservation (KMR), Hanapēpe Armory, Kekaha Firing Range (KFR), Pu‘unēnē Training Facility, Ukumehame Firing Range (UFR), Kaunakakai Armory, Bellows Regional Training Institute, Kalaeloa, Barber’s Point, and the 487th Military Parking Facility, Wahiawā. Seasonal frequency of bat detection was similar to previous acoustic studies for the islands of Hawai‘i, Maui, and O‘ahu. Hawaiian hoary bats were recorded at HIARNG installations during periods of pregnancy, lactation, and pup fledging. Our acoustic sampling did not record bat vocalizations at Fort Ruger and Waiawa Armory. Foraging activity was observed at nine acoustic monitoring stations on the islands of Hawai‘i, Kaua‘i, O‘ahu, and Maui. No foraging activity was observed on Moloka‘i and a single station on O‘ahu recorded one feeding buzz in September 2017 at Kalaeloa, Barber’s Point. Within-night detections showed bat activity was mostly confined to the first six hours of the night but was also variable among stations. In addition to long-term bat acoustic monitoring at HIARNG installations, Hawaiian hoary bat insect prey sampling with paired acoustic monitoring was conducted at KMR on Hawai‘i Island from May through August 2018. Insect abundance and bat activity were sampled within areas where goats and sheep were used to control weeds to determine if grazing by these ungulates attract and support potential prey for the insectivorous Hawaiian hoary bat. The assessment focused on types of flies that are often associated with livestock (muscoid flies, including house flies [Muscidae], blow flies [Calliphoridae], flesh flies [Sarcophagidae], and biting midges [Ceratopogonidae]), and moths that may be impacted by changes in the availability of grass. Insect abundance was found to vary in both space and time across the study area, with numbers of muscoid flies and biting midges increasing in the presence of livestock at some stations. Although these insects appeared to respond to livestock grazing in some instances, we did not find statistically significant responses in bat foraging as measured by echolocation activity between grazed stations and the ungrazed reference station. Thus, we found no evidence that suggested bats are drawn to foraging resources in grazed areas. This result may be influenced by several factors, including the size of Hawaiian hoary bat foraging ranges compared to the scale of study area, the type of ungulate and their dung, and the timing of insect activity. Hawaiian hoary bats use KMR and forage seasonally as evidenced by long-term acoustic studies and their presence in the ungulate grazing areas; however, the relatively small size of the ungulate herd and the area that they graze may not be able to support enough prey to have a significant influence on bat foraging rates.Item Hawaiian hoary bat acoustic monitoring on U. S. Army O‘ahu facilities.(2019-03-17) Bonaccorso, Frank J.; Montoya-Aiona, Kristina; Pinzari, Corinna A.Acoustic sampling for occurrence of the endangered Hawaiian hoary bat (Lasiurus cinereus semotus) was conducted at 12 locations on U. S. Army installations on O‘ahu Island, Hawai‘i. Bats were confirmed as present at 10 of these locations: Dillingham Military Reservation, Helemano Military Reservation, Kahuku Training Area, Kawailoa Training Area, Mākua Military Reservation, Schofield Barracks East Range, Schofield Barracks West Range, Schofield Barracks (Mendonca Park Housing), Tripler Army Medical Center, and Wheeler Army Airfield. Our acoustic sampling did not record bat vocalizations at Fort DeRussy or Fort Shafter. Despite the presence of bats at the above 10 locations, foraging activity as identified from characteristic feeding buzzes was observed only at East Range and West Range of Schofield Barracks. Nevertheless, Hawaiian hoary bats were recorded actively searching for prey in airspace at 10 of the 12 areas during important periods of Hawaiian hoary bat life history, including periods of pregnancy, lactation, and pup fledging. Within-night bat activity pooled for all nights and detectors at each location showed bat activity was mostly confined to the first several hours of the night. This acoustic study detected bats at lower rates of occurrence (frequency of detection [“f”] = 0.07) compared to detection probabilities (“dp”) observed on the islands of Hawai‘i (dp = 0.56) and Maui (dp = 0.27), implying either behavioral differences or that they occur at lower densities on O‘ahu. The rate is also consistent with results from two previous acoustic studies conducted on O‘ahu; a year long monitoring study in the northern Ko‘olau Mountains in 2014 (dp = 0.08), and short-term seasonal Army monitoring efforts in 2012 (dp = 0.05 to 0.06).