Evaluation of antioxidant and anti-cancer activities of Fucose-containing sulfated polysaccharide (FCSPs) from Hawaiian Marine Algae

Simple item page
dc.contributor.authorFukuchi, Ashley
dc.contributor.authorSang-ngern, Mayuramas
dc.contributor.authorSunada, Nathan
dc.contributor.authorPhosri, Santi
dc.contributor.authorTan, Ghee Teng
dc.contributor.authorChang, Leng Chee
dc.date.accessioned2018-04-03T19:45:41Z
dc.date.available2018-04-03T19:45:41Z
dc.date.issued2018-04-03
dc.descriptionStudent Research Poster, 2016
dc.description.abstractFucoidan is a group of sulfated polysaccharides isolated from marine algae. Fucoidan exhibits a variety of biological activities which are related to their chemically distinct sulfated fucose backbone. The structures of select fucoidans vary by species, and the structure and bioactivity remains unclear. The fucoidan of Padina sp., Asparagopsis taxiformus, and Nishime Kombu were extracted using four different methods using water at different temperatures. Method 2 gave the highest yield of fucoidan by 1H NMR analysis, whereas method 4 yielded FCSPs with anti-cancer activities. The crude fucoidan of Padina sp. was further purified using ion-exchange chromatography, resulting in the highest yield of fucoidan at 2.5 M NaCl. Cell viability was determined using sulforhodamine B (SRB) assay in five cancer cells (LNCap, PC-3, MCF-7, Caco-2, and LU-1). Both standard and Kombu-extracted fucoidan showed no effects on prostate (LNCaP and PC-3), and breast (MCF-7) cancer cells, but inhibited the growth of colon (Caco-2) and lung (LU-1) cancer cells by 43-74% at concentrations in the range of 50-400 µg/mL. These crude FCSPs also exhibited antioxidant activity. The FRAP values of Nishime Kombu, U-Fn, and Fucoidan Force, were 586.44 ± 107 µM/mg extract, 2922.38 ± 981 µM/mg, and 4212.03 ± 745 µM/mg, respectively.
dc.description.sponsorshipINBRE
dc.format.extent1 poster
dc.identifier.urihttp://hdl.handle.net/10790/3440
dc.language.isoen-US
dc.rightsCreative Commons
dc.subjectFucoidan
dc.subjectAsparagopsis taxiformus
dc.subjectPadina sp.
dc.subjectH NMR analysis
dc.subjectINBRE
dc.subjectHawaii
dc.titleEvaluation of antioxidant and anti-cancer activities of Fucose-containing sulfated polysaccharide (FCSPs) from Hawaiian Marine Algae
dc.typePresentation
dc.type.dcmiText

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Evaluation of antioxidant and anti-cancer activity of Fucose containing sulfated polysaccharide (FCPs) from Hawaiian Marine Algae.pdf
Size:
1.21 MB
Format:
Adobe Portable Document Format
Description:
Student Research Poster, INBRE 2016
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.62 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

DKICP Student Research Projects