Evaluation of antioxidant and anti-cancer activities of Fucose-containing sulfated polysaccharide (FCSPs) from Hawaiian Marine Algae

dc.contributor.author Fukuchi, Ashley
dc.contributor.author Sang-ngern, Mayuramas
dc.contributor.author Sunada, Nathan
dc.contributor.author Phosri, Santi
dc.contributor.author Tan, Ghee Teng
dc.contributor.author Chang, Leng Chee
dc.date.accessioned 2018-04-03T19:45:41Z
dc.date.available 2018-04-03T19:45:41Z
dc.date.issued 2018-04-03
dc.description Student Research Poster, 2016
dc.description.abstract Fucoidan is a group of sulfated polysaccharides isolated from marine algae. Fucoidan exhibits a variety of biological activities which are related to their chemically distinct sulfated fucose backbone. The structures of select fucoidans vary by species, and the structure and bioactivity remains unclear. The fucoidan of Padina sp., Asparagopsis taxiformus, and Nishime Kombu were extracted using four different methods using water at different temperatures. Method 2 gave the highest yield of fucoidan by 1H NMR analysis, whereas method 4 yielded FCSPs with anti-cancer activities. The crude fucoidan of Padina sp. was further purified using ion-exchange chromatography, resulting in the highest yield of fucoidan at 2.5 M NaCl. Cell viability was determined using sulforhodamine B (SRB) assay in five cancer cells (LNCap, PC-3, MCF-7, Caco-2, and LU-1). Both standard and Kombu-extracted fucoidan showed no effects on prostate (LNCaP and PC-3), and breast (MCF-7) cancer cells, but inhibited the growth of colon (Caco-2) and lung (LU-1) cancer cells by 43-74% at concentrations in the range of 50-400 µg/mL. These crude FCSPs also exhibited antioxidant activity. The FRAP values of Nishime Kombu, U-Fn, and Fucoidan Force, were 586.44 ± 107 µM/mg extract, 2922.38 ± 981 µM/mg, and 4212.03 ± 745 µM/mg, respectively.
dc.description.sponsorship INBRE
dc.format.extent 1 poster
dc.identifier.uri http://hdl.handle.net/10790/3440
dc.language.iso en-US
dc.rights Creative Commons
dc.subject Fucoidan
dc.subject Asparagopsis taxiformus
dc.subject Padina sp.
dc.subject H NMR analysis
dc.subject INBRE
dc.subject Hawaii
dc.title Evaluation of antioxidant and anti-cancer activities of Fucose-containing sulfated polysaccharide (FCSPs) from Hawaiian Marine Algae
dc.type Presentation
dc.type.dcmi Text
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Evaluation of antioxidant and anti-cancer activity of Fucose containing sulfated polysaccharide (FCPs) from Hawaiian Marine Algae.pdf
Size:
1.21 MB
Format:
Adobe Portable Document Format
Description:
Student Research Poster, INBRE 2016
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.62 KB
Format:
Item-specific license agreed upon to submission
Description: