Colorimetric determination of glyphosate in water using ozonolysis followed by spectrophotometric analysis of phosphate product with molybdenum blue reagent: a simple, fast and inexpensive assay of glyphosate.
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2022-12
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Abstract
A simple and inexpensive assay for quantifying glyphosate in aqueous environments using ozonolysis followed by spectrophotometric analysis of phosphate product with the molybdenum blue reagent has been developed. Glyphosate was oxidized with ozone gas in the presence of sodium hydroxide (1*10-3M) and then reacted with the molybdenum blue reagent to yield a colorimetrically quantifiable phosphate product. The absorbance of the heteropoly blue color complex was measured at 830 nm. The concentration of glyphosate was determined using a standard phosphate calibration curve concentration range from 10-4 to 10-8M in the presence of NaOH (1*10-3M). The reliable detection limit of glyphosate in aqueous environments when using ozonolysis and spectrophotometric methods was found to be 1*10-5M. The method developed in this study may be applicable in aquatic environments such as streams and estuaries and can be used by high school students, undergraduates, and field workers to obtain quantitative data.
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Chemistry, Analytical chemistry, Environmental science, Glyphosate, Molybdenum blue reagent, Ozonolysis, Spectrophotometry
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16 pages
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