Colorimetric determination of glyphosate in water using ozonolysis followed by spectrophotometric analysis of phosphate product with molybdenum blue reagent: a simple, fast and inexpensive assay of glyphosate.

dc.contributor.advisor Platz, Matthew
dc.contributor.author Criscione, Rose
dc.contributor.department Tropical Conservation Biology & Environmental Science
dc.date.accessioned 2023-02-03T00:39:48Z
dc.date.available 2023-02-03T00:39:48Z
dc.date.issued 2022-12
dc.description.degree M.S.
dc.identifier.uri http://hdl.handle.net/10790/7323
dc.subject Chemistry
dc.subject Analytical chemistry
dc.subject Environmental science
dc.subject Glyphosate
dc.subject Molybdenum blue reagent
dc.subject Ozonolysis
dc.subject Spectrophotometry
dc.title Colorimetric determination of glyphosate in water using ozonolysis followed by spectrophotometric analysis of phosphate product with molybdenum blue reagent: a simple, fast and inexpensive assay of glyphosate.
dc.type Thesis
dcterms.abstract A simple and inexpensive assay for quantifying glyphosate in aqueous environments using ozonolysis followed by spectrophotometric analysis of phosphate product with the molybdenum blue reagent has been developed. Glyphosate was oxidized with ozone gas in the presence of sodium hydroxide (1*10-3M) and then reacted with the molybdenum blue reagent to yield a colorimetrically quantifiable phosphate product. The absorbance of the heteropoly blue color complex was measured at 830 nm. The concentration of glyphosate was determined using a standard phosphate calibration curve concentration range from 10-4 to 10-8M in the presence of NaOH (1*10-3M). The reliable detection limit of glyphosate in aqueous environments when using ozonolysis and spectrophotometric methods was found to be 1*10-5M. The method developed in this study may be applicable in aquatic environments such as streams and estuaries and can be used by high school students, undergraduates, and field workers to obtain quantitative data.
dcterms.extent 16 pages
dcterms.language en
dcterms.publisher University of Hawaii at Hilo
dcterms.rights All UHH dissertations and theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission from the copyright owner.
dcterms.type Text
local.identifier.alturi http://dissertations.umi.com/hilo.hawaii:10231
Files
Original bundle
Now showing 1 - 3 of 3
Thumbnail Image
Name:
Criscione_hilo.hawaii_1418O_10231.pdf
Size:
718.93 KB
Format:
Adobe Portable Document Format
Description:
Thumbnail Image
Name:
Criscione_Appendix1.pdf
Size:
405.96 KB
Format:
Adobe Portable Document Format
Description:
Thumbnail Image
Name:
Criscione_Appendix2.pdf
Size:
345.15 KB
Format:
Adobe Portable Document Format
Description: